(A) γ-Irradiated 6-week-old WT and GARKO mice were adoptively transplanted with WT or GARKO bone marrow cells. To confirm the bone marrow engraftment, various tissues from a WT recipient receiving GARKO bone marrow were subjected to AR genotyping. KO, truncated AR allele. (B) After bone marrow engraftment, wounds were created and the wound areas were quantified and compared among recipients. n = 3–4 (6 wounds/mouse). The numbers indicate the P values for each comparison. (C) Various tissues from MARKO mice were subjected to AR genotyping to confirm the efficiency and specificity of AR deletion. D3W, day 3 wound; Thy, thymus; Spln, spleen; Mus, muscle; Tes, testis; Bld, bladder. (D) Wound areas were compared between male MARKO mice and their WT littermates. n = 6–7 (2 wounds/mouse). (E) Day 8 wound tissues from WT and MARKO mice were subjected to Trichrome staining to detect collagen fibers (blue). Ovals indicate granulation tissues. Scale bars: 500 μm. The quantitative data show relative collagen deposition in granulation tissues compared with the adjacent dermis. n = 3 (4 wounds/mouse). (F) Day 3 wound tissues from WT and MARKO mice were subjected to H&E staining for analysis of re-epithelialization. n = 4 (6 wounds/mouse). (G) Excisional wounds were created and compared between KARKO mice and their WT littermates. n = 3–4 (6 wounds/mouse). (H) Excisional wounds were created and compared between FARKO mice and their WT littermates. n = 4–6 (6 wounds/mouse). Due to differences in genetic backgrounds among conditional ARKO lines, the WT controls among different experiments were not necessarily equivalent.