JCI - Mammalian target of rapamycin regulates murine and human cell differentiation through STAT3/p63/Jagged/Notch cascade

Mammalian target of rapamycin regulates murine and human cell differentiation through STAT3/p63/Jagged/Notch cascade

Jianhui Ma, Yan Meng, David J. Kwiatkowski, Xinxin Chen, Haiyong Peng, Qian Sun, Xiaojun Zha, Fang Wang, Ying Wang, Yanling Jing, Shu Zhang, Rongrong Chen, Lianmei Wang, Erxi Wu, Guifang Cai, Izabela Malinowska-Kolodziej, Qi Liao, Yuqin Liu, Yi Zhao, Qiang Sun, Kaifeng Xu, Jianwu Dai, Jiahuai Han, Lizi Wu, Robert Chunhua Zhao, Huangxuan Shen, Hongbing Zhang

Published in Volume 120, Issue 1

J Clin Invest. 2010; 120(1):103–114 doi:10.1172/JCI37964

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Figure 10

NF-κB and STAT3/p63 control Notch signaling downstream of mTORC1 in parallel.

(A) WT and Tsc2^–/– MEFs were treated with or without 10 nM rapamycin for 24 hours and then subjected to immunoblotting for NF-κB (p65), IκBα, and mTOR signaling. (B) Tsc2^–/– MEFs were transfected with p65 siRNA or STAT3 siRNA to knock down p65 or STAT3 expression for 48 hours and were then subjected to immunoblotting for STAT3 and NF-κB signaling. Non–target-directed random siRNA served as a control. (C) Schematic illustration of how NF-κB and STAT3/p63 regulate Notch signaling downstream of mTORC1 in parallel.