(A) Shown are urinary albumin excretion (left) and the extent of glomerulosclerosis (right) 30 days after administration of anti-podo Abs in individual Daf^–/– mice depleted of CD4⁺ cells, with mAb GK1.5 or controls receiving irrelevant rat IgG_2b. All data were normally distributed (Anderson-Darling test; P > 0.05) and compared by t testing, with P values provided above the data. (B) Infiltration with Thy-1.2⁺ T cells (brown, arrow) and F4/80⁺ monocytic cells (purple, arrow) was significantly reduced 30 days after administration of anti-podo Abs in CD4⁺ cell–depleted but not control Daf^–/– mice. (C) Balb/c^nu/nu mice reconstituted with DAF-deficient but not WT T cells developed albuminuria and FSGS. Urinary albumin excretion (left) and the percentage of segmentally sclerotic glomeruli (right) in individual Balb/c^nu/nu mice that received T cells from WT (blue symbols) or Daf^–/– mice (red symbols) 30 days after administration of anti-podo Abs. Experiments differed by whether mice were reconstituted with partially (diamonds) or highly (circles) purified T cells. All data sets in mice receiving T cells from Daf^–/– mice were normally distributed with no differences comparing partially and highly purified T cells. Means and 95% CIs for the combined groups are shown as horizontal bars and open boxes, respectively. (D) Representative PAS-stained kidney sections from individual mice show the presence of segmental sclerosis (arrow) only in mice with DAF-deficient T cells. Original magnification, ×600.