Figure 3
Cardiac defects resulting from cardiac deletion of
Hdac3.
(A) HW/BW ratios of WT and Hdac3cko showing progression of cardiac hypertrophy. (B) Kaplan-Meier survival curve showing lethality by 16 weeks in Hdac3cko mice. (C) Masson trichrome–stained sections of wild-type and Hdac3cko mice at 12 weeks. Deletion of Hdac3 results in cardiac hypertrophy, left atrial thrombus, and cardiac fibrosis, seen in blue. Original magnification, ×20 (lower panels). (D) Expression of cardiac stress markers in Hdac3cko mice at 8 weeks. mRNA transcript levels were detected by real-time RT-PCR and normalized to 18S ribosomal RNA. ANF, atrial natriuretic factor; BNF, brain natriuretic peptide; αSkActin, α-skeletal actin. (E) Electron microscopy of LV tissue from WT and Hdac3cko hearts at 8 weeks. Scale bars: 5000 nm (×4,200); 1000 nm (×16,500).
