(A) Semiquantitative RT-PCR showing Hdac3 transcript levels in wild-type and Hdac3cko mice using primers in exon 10, forward (F), and exon 15, reverse (R), or exon 13, forward, and exon 15, reverse. Cardiac deletion of Hdac3 results in deletion of exons 11 through 14. (B) Western blot showing reduced expression of Hdac3 in Hdac3cko hearts. (C) Real-time PCR of class I and class II Hdacs in wild-type and Hdac3cko hearts. cKO, Hdac3cko.