Figure 1
Hsp104 reduces dopaminergic cell loss in the substantia nigra of rats expressing human α-syn A30P.
(A) Staining for TH at 6 weeks in the substantia nigra of rats injected with either lenti-A30P/lenti-YFP or lenti-A30P/lenti-Hsp104. (B) Quantitation of TH-IR nigral neurons at 6 weeks relative to the contralateral side in rats unilaterally injected with the different lentiviral constructs. Values represent mean ± SEM; n = 7 animals for both A30P/YFP and A30P/Hsp104 groups; P < 0.05. (C) Confocal imaging of triple labeling for TH (red), A30P human α-syn (blue), and Hsp104 (green) in the rat substantia nigra injected with lenti-A30P/lenti-Hsp104. Higher magnification (bottom row) reveals numerous TH neurons positive for Hsp104 and A30P human α-syn at 6 weeks after injection. Scale bar: 350 μm (A and C, top row); 60 μm (C, bottom row).
