(A) CCND2 expression analysis in P7 blast cells compared with a series of primary T-ALL cases from different oncogenic subgroups — CCND2-rearranged cases (TCR-CCND2; n = 3) and other T-ALL primary cases (T-ALL; n = 86) — and with normal thymus (n = 3). CCND2 expression levels are shown as copy number value in a given sample related to TBP copy number in the series, as determined using the ΔCt method. CCND2 levels in 11 normal human thymic subpopulations were previously analyzed for comparison and showed varied gene expression depending on the cells’ stage of thymic differentiation (18). Results are presented as a box plot: the box boundaries indicates the twenty-fifth and seventy-fifth percentiles; the line within the box denotes the median; whiskers denote tenth and ninetieth percentiles; outliers are indicated as dots. (B) RQ-PCR of SPAG6, BMI1, COMMD3, and DNAJC1 genes in P10 leukemic sample at M+33 compared with CALM-AF10–positive (CA+; n = 14) and –negative (CA–; n = 31) samples. The ratio of gene expression relative to ABL was calculated for each sample. Below, the genomic organization around the AF10 breakpoint is presented along with the location of the γc retrovirus insertion. (C) Longitudinal expression of LMO2 in P10 as determined by RQ-PCR.