Neural stem cell transplantation can ameliorate the phenotype of a mouse model of spinal muscular atrophy
J. Clin. Invest. 118:10 doi:10.1172/JCI35432 [Go to this article.]

Figure 1
ALDH^hiSSC^lo cells can differentiate into motoneurons in vitro.

After in vitro differentiation, ALDH^hiSSC^lo cells derived from HB9-GFP transgenic mice that express GFP protein (green) only in motoneurons give rise to neurons with a complex morphology (A and B), showing a neuronal and motoneuronal phenotype confirmed by the expression of neuronal marker TuJ1 (C, D, and merged image in E), ChAT (merged images for HB9-GFP and ChAT; F and G), and islet-1 (H and I, merge). Nuclei are stained with DAPI. Scale bars: 70 μm (A); 50 μm (B and F–I); 100 μm (C–E).