Figure 5
qPCR validation of selected microarray gene-expression measurements.
(A) Heat map representing the expression profiles of genes with significant differential expression when comparing TOL with non-TOL and TOL with CONT samples (t test; P < 0.05). The intensity of each color denotes the standardized ratio between each value and the average expression of each gene across all samples. Red pixels correspond to an increased abundance of mRNA in the indicated blood sample, whereas green pixels indicate decreased mRNA levels. The checkerboard plot on the left represents the statistical significance of TOL versus non-TOL and TOL versus CONT comparisons, with black squares corresponding to P < 0.05 by t test. (B) Multidimensional scaling plot incorporating TOL (triangles), non-TOL (circles), and CONT (filled) samples. Distances between samples plotted in the 3D graph are proportional to their dissimilarities in gene expression as assessed by qPCR. CONT samples cluster between TOL and non-TOL samples.
