Figure 1
Arsenite-stimulated defenestration and capillarization in vivo and ex vivo.
(A) Representative scanning EM images were captured from liver sections of control mice or mice exposed for 2 wk to 10–250 ppb arsenite in their drinking water. Morphometric analysis was used to calculate porosity (percent of open space in the fenestrations relative to area of vessel wall) from 10 midlobular sinusoid vessel images from each of 3 separate mice per treatment group. Data are mean ± SD, with significance of differences determined by ANOVA followed by Dunnet’s post test (n = 3 mice per treatment). (B) Primary cultured SECs were exposed ex vivo to arsenite at the indicated concentrations for 8 h. Cells were fixed and processed for scanning EM imaging at ×10,000 magnification. Data are mean ± SD from analysis of 5 images from 5 individual coverslips of cells per treatment (n = 4 mice per treatment). **P < 0.01 versus control. Scale bars: 1 μm.
