DNA damage induced by chronic inflammation contributes to colon carcinogenesis in mice
J. Clin. Invest. Lisiane B. Meira, et al. 118:2516 doi:10.1172/JCI35073 [
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Figure 3Increased severity of disease in
Aag–/– versus
Aag+/+ animals treated with 7 cycles of DSS.
(
A) Treatment scheme. (
B) Decrease in colon length for
Aag–/– animals (
n = 18) compared with
Aag+/+ animals (
n = 7). (
C) Increase in spleen weight as a percentage of body weight for
Aag+/+ (
n = 7) and
Aag–/– animals (
n = 18). Data are mean ± SD. (
D) Pathology scores for
Aag+/+ (circles) and
Aag–/– (diamonds) mice. (
E) Histopathology of colonic disease induced by 7 cycles of DSS. From left to right:
Aag+/+ colon exhibited moderate inflammation and glandular epithelial hyperplasia;
Aag+/+ colon exhibited mild dysplasia characterized by epithelial cell pleomorphism and mild branching, with hyperplasia and inflammation;
Aag–/– colon exhibited moderate to severe inflammation, crypt atrophy, mucosal collapse, and segmental epithelial cell loss;
Aag–/– colon exhibited a portion of an intraepithelial neoplasia with mucosal dysplasia characterized by loss of columnar orientation, elongation, branching and infolding, glandular ectasia, inflammation, and crypt abscesses. Scale bars: 100 μm.
