Lisiane B. Meira, James M. Bugni, Stephanie L. Green, Chung-Wei Lee, Bo Pang, Diana Borenshtein, Barry H. Rickman, Arlin B. Rogers, Catherine A. Moroski-Erkul, Jose L. McFaline, David B. Schauer, Peter C. Dedon, James G. Fox, Leona D. Samson
Increased tumor multiplicity in Aag–/– versus Aag+/+ animals treated with AOM and DSS.
(A) Treatment scheme. White blocks represent 16 days of normal water except the last which is 7 days. Black blocks represent 5 days of 2.5% DSS in water except for the last, which was 4 days of 2% DSS. Mice were also treated with only AOM or only DSS. (B) Tumor multiplicity in Aag+/+ animals (white), and Aag–/– animals (black). AOM+DSS Aag+/+, n = 12; AOM+DSS Aag–/–, n = 23; AOM Aag+/+, n = 15; AOM Aag–/–, n = 19; DSS Aag+/+, n = 10; and DSS Aag–/–, n = 10. Data are mean ± SD. (C) Pathology scores for Aag+/+ (circles) and Aag–/– (diamonds) mice. See Supplemental Methods for a complete description of scoring criteria. Lines indicate the median. (D) Photomicrographs depicting, from left to right, normal untreated Aag+/+ colon; AOM+DSS-treated Aag+/+ colon bearing a lesion with an average dysplasia score, a sessile adenoma; normal untreated Aag–/– colon; and AOM+DSS-treated Aag–/– colon bearing a lesion with an average dysplasia score and a higher scoring pedunculated adenoma. Scale bars: 200 μm.