DNA damage induced by chronic inflammation contributes to colon carcinogenesis in mice
J. Clin. Invest. Lisiane B. Meira, et al. 118:2516 doi:10.1172/JCI35073 [
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Figure 1Increased tumor multiplicity in
Aag–/– versus
Aag+/+ animals treated with AOM and DSS.
(
A) Treatment scheme. White blocks represent 16 days of normal water except the last which is 7 days. Black blocks represent 5 days of 2.5% DSS in water except for the last, which was 4 days of 2% DSS. Mice were also treated with only AOM or only DSS. (
B) Tumor multiplicity in
Aag+/+ animals (white), and
Aag–/– animals (black). AOM+DSS
Aag+/+,
n = 12; AOM+DSS
Aag–/–,
n = 23; AOM
Aag+/+,
n = 15; AOM
Aag–/–,
n = 19; DSS
Aag+/+,
n = 10; and DSS
Aag–/–,
n = 10. Data are mean ± SD. (
C) Pathology scores for
Aag+/+ (circles) and
Aag–/– (diamonds) mice. See Supplemental Methods for a complete description of scoring criteria. Lines indicate the median. (
D) Photomicrographs depicting, from left to right, normal untreated
Aag+/+ colon; AOM+DSS-treated
Aag+/+ colon bearing a lesion with an average dysplasia score, a sessile adenoma; normal untreated
Aag–/– colon; and AOM+DSS-treated
Aag–/– colon bearing a lesion with an average dysplasia score and a higher scoring pedunculated adenoma. Scale bars: 200 μm.
