Low-field paramagnetic resonance imaging of tumor oxygenation and glycolytic activity in mice
J. Clin. Invest. Shingo Matsumoto, et al. 118:1965 doi:10.1172/JCI34928 [
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Figure 1The pulsed EPR system. (
A) EPRI spectrometer used for pO
2 imaging. Oxygen imaging is carried out using the 300 MHz pulsed EPR scanner operating at 10 mT, having 5 ns time resolution for signal detection. (
B) A 4D (3D spatial and 1D spectral) EPR image was obtained after infusion of the oxygen sensitive probe TAM equilibrated at different levels of oxygen as indicated in the figure. Spatially resolved EPR line width of TAM can be extracted from the EPR signal intensity image, which has linear relationship with partial pressure of oxygen (
C), and consequently, an absolute pO
2 map (
D) can be obtained with anatomical overlay by sequential MRI scan of the same object.
