(A) qRT-PCR analysis of pBABE and KLF6-SV1 vector–retrovirally infected PC3 and P3M cell lines demonstrated 12- and 27-fold overexpression, respectively, of KLF6-SV1 in pBABE-SV1 vector–infected cell lines compared with control. (B) KLF6-SV1–overexpressing cell lines proliferated significantly more than control cell lines, assessed by tritiated thymidine incorporation at 24 and 72 h of the PC3 and PC3M cell lines. Mean change in cell growth rate from 3 independent experiments is shown. (C) Increased expression of the oncogene c-myc and antiapoptotic Bcl-2, with concomitant reduction of the cyclin-dependent inhibitor p21, in KLF6-SV1–expressing cell lines. RNA and protein were harvested from 3 independent experiments, and qRT-PCR and Western blotting were performed. Overexpression of KLF6-SV1 increased Bcl-2 expression 50%, increased c-myc expression 80%, and reduced p21 expression 50% in PCa cell lines at the mRNA and protein levels. Actin was used as loading control for KLF6-SV1 and p21; tubulin was used for c-myc. (D) Increased invasion in KLF6-SV1 cell lines was associated with increased expression of MMP9. Gelatin zymography of the supernatant isolated from PC3 and PC3M cell lines is shown. Overexpression of KLF6-SV1 increased MMP9 expression in both PC3 and PC3M lines. KLF6-SV1–expressing stable PC3 cell lines (PC3-SV1) were 2-fold more invasive through a Matrigel basement membrane. The number of invasive cells was counted from 4 fields from 3 independent experiments. **P < 0.01, ***P < 0.001 versus control.