(A) RT-PCR of representative prostate-derived cDNAs with KLF6-specific primers. N, normal prostate; L, localized PCa; M, metastatic PCa. (B) qRT-PCR analysis of localized and metastatic PCa cDNAs for wild-type KLF6 expression. Metastatic tumors expressed significantly less wild-type KLF6 mRNA compared with localized tumors. **P < 0.001. (C) qRT-PCR of localized and metastatic PCa samples using wild-type KLF6– and KLF6-SV1–specific PCR primers (refs. 14, 15, and our unpublished observations). (D) Increased KLF6-SV1 expression in metastatic PCa. Western blot analysis using a KLF6-SV1–specific monoclonal antibody (ref. 15 and our unpublished observations). Transfected KLF6-SV1 and transfected wild-type KLF6 controls were run on the same gel but were noncontiguous. Tubulin was used as the loading control for all lanes. (E) Left: DNA microarray analysis of PCa demonstrated downregulation of KLF6 mRNA in hormone-refractory metastatic PCa (HR-MET) compared with both noncancerous prostate tissue and localized PCa. NAP, normal adjacent prostate tissue; PCA, localized PCa. Right: Tissue microarray analysis of KLF6 expression using KLF6 monoclonal antibody 2A2. Data points and error bars represent mean KLF6 protein expression and 95% confidence intervals, respectively. (F) Immunohistochemistry of high-density tissue microarray analyses with the KLF6-SV1–specific monoclonal antibody demonstrated marked upregulation of KLF6-SV1 expression in hormone-refractory metastatic PCa compared with naive metastatic PCa, localized PCa, and benign prostate tissue (P < 0.001). Data points and error bars represent mean KLF6-SV1 protein expression and 95% confidence intervals, respectively. (G) Median survival, as measured using biochemical recurrence and assessed by qRT-PCR, in men whose localized prostate tumors expressed high levels of KLF6-SV1 (blue) was 30 mo compared with 80 mo in men with low KLF6-SV1–expressing tumors (P < 0.01).