Figure 2
HDAC inhibition modulates allogeneic T cell proliferation and IL-2 production in vitro.
SAHA was added at the indicated concentrations to DCs isolated from C57BL/6 BM that were then immediately used as stimulators in an MLR with T cells from either BALB/c (allogeneic) or C57BL/6 (syngeneic) mice as described in Methods. (A) T cell proliferation was determined by 3H-thymidine incorporation at 72 h. Allogeneic T cell responses from control (0 μM SAHA) and SAHA-treated cultures and syngeneic T cell responses to control DCs (syn) are shown. Data are mean ± SEM of quadruplicate cultures. P = NS, control versus 0.5-μM SAHA. **P < 0.03 versus control. Results are from 1 of 3 similar experiments. (B) Supernatants of cultures with control (open symbols) or SAHA (filled symbols) were collected at 48 h, and IL-2 was measured by ELISA. Data are mean ± SEM of quadruplicate cultures. *P < 0.05 versus control. (C) B6BMDCs were pretreated with diluent or 500 nM SAHA for 16–18 h, washed, and used as stimulators with allogeneic BALB/c T cells at the indicated ratios. T cell proliferation was evaluated after 72 h of culture.
