Hypertensive encephalopathy and the blood-brain barrier: is δPKC a gatekeeper?
J. Clin. Invest. Wen-Hai Chou, et al. 118:17 doi:10.1172/JCI34516 [Go to this article.]

Figure 2
The chemical genetics approach. An as-kinase with a hole (in blue) recognizes the N6-(benzyl)-ATP-γS (BZ-ATPγS) with a bump and phosphorylates its unique substrates. Only the as-kinase, not wild-type kinases (in brown), can use N6-substituted ATP analogs (BZ-ATPγS) as phosphate donors. In the second step, alkylation with p-nitrobenzylmesylate (PNBM) forms thiophosphate esters and thioethers. Only esterified as-kinase substrates are recognized by specific antibodies discriminating thiophosphate esters from thioethers.