Kidney injury molecule–1 is a phosphatidylserine receptor that confers a phagocytic phenotype on epithelial cells
J. Clin. Invest. Takaharu Ichimura, et al. 118:1657 doi:10.1172/JCI34487 [
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Figure 7Macrophages do not express Kim-1/Tim-1. RT-PCR (left panel) for
Kim1 mRNA from day 7 mouse bone marrow macrophages cultured with LPS or dexamethasone.
Emr1 (F4/80 antigen) and
Gapdh were used as controls, and mouse kidney cDNA 48 hours following ischemia was used as a positive control. Immunoblot (right panel) for Kim-1 in protein lysates from cultured macrophages and postischemic mouse kidney. Note that mouse bone marrow–derived macrophages generate neither
kim1 transcript nor Kim-1 protein, in quiescent or activated states. BMMf, bone marrow–derived macrophages.
