Regression of human kidney cancer following allogeneic stem cell transplantation is associated with recognition of an HERV-E antigen by T cells
J. Clin. Invest. Yoshiyuki Takahashi, et al. 118:1099 doi:10.1172/JCI34409 [Go to this article.]

Figure 7
Expression of CT-RCC-8 and CT-RCC-9 in tumors and nonmalignant tissues. CT-RCC-8 and CT-RCC -9 were found to be expressed only in RCC cells and not a variety of different non-RCC tumor cell lines or in any normal tissues. (A) Semiquantitative RT-PCR for CT-RCC-8 and -9 was performed using cDNAs prepared from 14 different human RCC tumor lines. Both CT-RCC-8 and -9 were detected in 8 of 14 RCC cell lines. (B) CT-RCC common region transcripts were detectable in the same 8 RCC cell lines at variable levels by quantitative real-time RT-PCR analysis using primers and probes specific to CT-RCC common region. (C) Lack of expression of CT-RCC-8 and -9 transcripts by RT-PCR in a variety of different non-RCC malignant cell lines. (D) Neither transcript was detected by RT-PCR in pooled cDNAs obtained from 24 normal human tissues, including the kidney and testis. β-Actin was used as an internal control, and cDNA from SAUJ-RCC was used as a positive control.