High incidence of leukemia in large animals after stem cell gene therapy with a HOXB4-expressing retroviral vector
J. Clin. Invest. Xiao-Bing Zhang, et al. 118:1502 doi:10.1172/JCI34371 [Go to this article.]

Figure 5
Characterization of macaque K03290, an animal with normal hematopoiesis. (A) Marking levels of K03290 in peripheral blood, which had been stabilized for more than 2 years. (B) Southern blot analysis showed multiple bands of HOXB4-overexpressing marrow cells. Digestion with SacI, which cut the transgene twice, showed a 3.9-kb band for all the integrants. BglII and EcoRI cut the transgene once, and so each band indicates an individual integrant.