ChREBP, but not LXRs, is required for the induction of glucose-regulated genes in mouse liver
J. Clin. Invest. Pierre-Damien Denechaud, et al. 118:956 doi:10.1172/JCI34314 [Go to this article.]

Figure 1
Glucagon injection in vivo promotes ChREBP nuclear delocalization and its phosphorylation on Ser196. C57BL/6J mice were fasted overnight and refed on a HCHO diet for 18h. (A) Total, Ser196 phosphorylated, and nuclear ChREBP protein in liver extracts from HCHO-refed mice treated with either NaCl or glucagon (0.5 U/kg) for 30 min. Lamin A/C antibody was used as a loading control. (B) ChREBP immunofluorescence analysis in liver sections from HCHO refed mice treated with either NaCl or glucagon (0.5 U/kg) for 30 min. Original magnification, ×400. n = 6 per group. No signal was obtained when liver sections were incubated with the secondary antibody only (data not shown).