Figure 2
Dermal-epidermal separation in the
Col7a1flNeo/flNeo mouse is caused by strongly reduced collagen VII deposition.
(A) Histological analysis (H&E staining) of paw skin of 1-day-old Col7a1WT/WT and Col7a1flNeo/flNeo mice shows dermal-epidermal blister formation (asterisk) with erythrocyte accumulation in the latter. (B) Indirect immunofluorescence signal of collagen VII (green) in paw skin of 1-day-old Col7a1flNeo/flNeo mice was strongly reduced compared with Col7a1WT/WT mice. Asterisk indicates the blister cavity. Scale bars: 50 μm. (C and D) Ultrastructural analysis showed normal hemidesmosomes (white arrows), but few anchoring fibrils (black arrows), in the skin of Col7a1flNeo/flNeo mice. Asterisk indicates the blister cavity. Original magnification, ×195,000.