Figure 4
Lithium enhances NHEJ repair in HT-22 hippocampal neurons.
(A) Schematic of the NHEJ repair assay. (B) Rejoining capacity of HT-22 neurons with or without lithium. The plasmid substrate pEGFP-N1 was linearized with the EcoRI endonuclease, which cleaves between the promoter and the GFP gene and prevents GFP expression. HT-22 cells were cotransfected with this linearized plasmid as well as the circular pdsRed as an internal control for transfection efficiency. At 48 hours after transfection, cells were harvested for 2-color flow cytometric analysis. Data (mean ± SEM from 3 independent experiments) show the ratio of GFP expression to pdsRed expression as a function of vehicle-treated cells. **P < 0.01 versus control.
