Figure 2
Lithium decreases the levels of persistent IR-induced γ-H2AX foci.
Cells were treated with 3 mM lithium for 7 days. Following the treatment period, cells were exposed to 3 Gy. After the indicated times, cells were processed for immunofluorescence staining for γ-H2AX. Data (mean ± SEM from at least 3 independent experiments) show the percentage of cells containing greater than 10 foci or the number of foci per cell, as indicated. (A) Percentage of cells with γ-H2AX foci in HT-22 mouse hippocampal neurons. (B) Representative foci staining in HT-22 cells. Original magnification, ×400. (C) The upper panel shows representative γ-H2AX foci immunofluorescence staining in irradiated and unirradiated mouse primary neurons. The lower panel shows the percentage of cells with γ-H2AX foci in mouse primary neurons. Original magnification, ×400. (D) Number of IR-induced γ-H2AX foci per cell, which was decreased by lithium treatment. *P < 0.05, **P < 0.01 versus control.
