Regulation of myeloproliferation and M2 macrophage programming in mice by Lyn/Hck, SHIP, and Stat5
J. Clin. Invest. Wenbin Xiao, et al. 118:924 doi:10.1172/JCI34013 [Go to this article.]

Figure 2
The MPD phenotype in Lyn/Hck-deficient mice. (A and B) Increased myeloid cells (Gr-1⁺CD11b⁺ or CD11b⁺) were found in BM, spleen (SPL), and peripheral blood (PB) from dko mice, as quantified by flow cytometry. (C) Shown is a Wright-Giemsa–stained blood smear of dko mouse (original magnification, ×400). Arrows indicate neutrophils. (D) Accumulated flow cytometric data of BM cells are shown (n = 12). Numbers of specific cell types were calculated by multiplying their percentages (obtained by flow cytometry; see Supplemental Figure 4) by total cell numbers. Dko mice have increased numbers of HSCs and myeloid progenitors but decreased numbers of lymphoid progenitors (see Supplemental Figures 4 and 5). MEP, megakaryocyte-erythroid progenitor.