The neglected role of antibody in protection against bacteremia caused by nontyphoidal strains of Salmonella in African children
J. Clin. Invest. Calman A. MacLennan, et al. 118:1553 doi:10.1172/JCI33998 [
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Figure 2Killing of NTS by blood and serum from Malawian adults and by C9-deficient serum at 45, 90, and 180 minutes. (
A) Killing of 8 invasive Malawian
S. Typhimurium isolates by whole blood from a healthy adult Malawian donor. (
B) Killing of
S. Typhimurium isolate D23580 by whole blood (squares), serum (circles), and blood cells washed in RPMI to remove antibody and complement (triangles). (
C) Effect of heat inactivation of serum on ability to kill D23580: fresh serum (circles), heat-inactivated serum (squares), and heat-inactivated serum reconstitution with lyophilized complement (triangles). (
D) Inability of C9-deficient serum (triangles) to kill D23580. Killing of
Salmonella was enabled by addition of exogenous C9 (squares). (
E) Killing of D23580 by fresh and heat-inactivated serum: 100% fresh (triangles); 60% fresh, 40% heat-inactivated (diamonds); 20% fresh, 80% heat-inactivated (squares); 10% fresh, 90% heat-inactivated (circles); and 100% heat-inactivated (x). Negative values correspond with a decrease in viable salmonellae compared with the initial concentration of 10
6 salmonellae/ml. In
A,
B,
C, and
E, data indicate blood and/or serum from 1 healthy adult and are representative of 4 healthy adult donors tested. Where error bars are present, data are mean ± 1 SD of 3 experiments.
