Mouse ES cell–derived cardiac precursor cells are multipotent and facilitate identification of novel cardiac genes
J. Clin. Invest. 118:3 doi:10.1172/JCI33942 [Go to this article.]

Figure 2
Cardiac marker expression in mES cell–derived GFP⁺ CPCs and efficiency of CPC derivation.

(A–D) Cardiac proteins Nkx2-5 (A), Myocd (B), Bmp2/4 (C), and Gata5 (D; all green) are coexpressed in cells expressing GFP (red) on differentiation day 6. Nuclei were stained with DAPI (blue). Scale bars: 100 μm. (E) Differentiating EBs were dissociated in single-cell suspensions at the indicated time points and the percentage of GFP⁺ cells was measured. Cells from dissociated EBs expressing GFP are shown in green; negative control sample is shown in red. Cells below the black line are GFP⁺ CPCs. (F) Efficiency of mES cell differentiation into CPCs, as quantified by FACS analysis for the percentage of GFP⁺ cells on differentiation days 5–10. (G) Number of GFP⁺ CPCs per 100 EBs on differentiation days 5–10. Error bars indicate SD.