Mutation of the Cyba gene encoding p22^phox causes vestibular and immune defects in mice
J. Clin. Invest. Yoko Nakano, et al. 118:1176 doi:10.1172/JCI33835 [Go to this article.]

Figure 6
The Cyba transgene reduces the B. cepacia susceptibility of the nmf333 strain. (A) The Cyba transgene consists of a CMV immediate-early enhancer, a chicken β-actin promoter, a chimeric intron, the p22phox-encoding Cyba sequence, and an SV40 polyadenylation (polyA) site. (B) Blue colorimetric test for superoxide production in PMA-stimulated neutrophil granulocytes isolated from homozygous nmf333 mice and from Cyba-transgenic homozygous nmf333 [Tg(Cyba)-nmf333] mice. Two neutrophils are visible in each. Nuclei were stained with Safranin O. Original magnification, ×63. Images are representative of n = 38. (C) Quantitative analysis of superoxide production by PMA-activated neutrophil granulocytes isolated from homozygous nmf333 mice, Cyba-transgenic homozygous nmf333, and WT mice. Superoxide production was assessed using the superoxide dismutase–sensitive ferricytochrome c reduction assay. Horizontal lines indicate the mean rate of superoxide production; each circle represents a single mouse (n = 5 to 8 mice). One-way ANOVA, P < 0.0001. post hoc Tukey’s test: *P < 0.05, **P < 0.01. (D) Probability of survival of homozygous nmf333 mice (filled squares; n = 5) and of Cyba-transgenic homozygous nmf333 mice (open triangles; n = 7) after intratracheal inoculation with 10⁴ CFU B. cepacia (log-rank test, P = 0.0004). (E) Typical histological appearance of lung sections prepared from Cyba-transgenic homozygous nmf333 mice 3.5 days after intratracheal inoculation with 10⁴ CFU B. cepacia (n = 3). Left: low-magnification overview. Right: higher-magnification view of peribronchial inflammation in the boxed region. Scale bars: 100 μm.