Mutation of the Cyba gene encoding p22^phox causes vestibular and immune defects in mice
J. Clin. Invest. Yoko Nakano, et al. 118:1176 doi:10.1172/JCI33835 [Go to this article.]

Figure 5
Expression of p22phox in the apical membrane of endolymphatic duct and sac. (A) Endolymphatic duct sections of a WT and a homozygous nmf333 mouse at E14 were immunostained with an anti-p22phox antibody and visualized by confocal microscopy. Top: localization of the immunofluorescence signal (p22phox). Bottom: superimposition of the fluorescence signal and the differential interference contrast image (merged). Asterisks indicate endolymphatic duct lumens. (B) Immunostaining of WT endolymphatic sac (ES) and duct (asterisk) with the anti-p22phox antibody at E17.5. Top: red fluorescence signal of p22phox immunolocalization. Bottom: superimposition of the fluorescence signal and the differential interference contrast image (merged). (C) In P1 WT mice, the lower region of the endolymphatic duct exhibits p22phox immunofluorescence (left). Right: both the red fluorescence signal and the differential interference contrast image. The asterisk indicates the lumen of the endolymphatic duct. Scale bars: 20 μm.