Mutation of the Cyba gene encoding p22^phox causes vestibular and immune defects in mice
J. Clin. Invest. Yoko Nakano, et al. 118:1176 doi:10.1172/JCI33835 [Go to this article.]

Figure 3
Necrotizing pneumonia in homozygous nmf333 mice following B. cepacia lung infection. (A) Probability of survival after inoculation with various CFU of B. cepacia (10⁴ CFU, filled squares; 10⁵ CFU, filled triangles; 10⁶ CFU, open circles and filled circles) into the tracheas of WT (open circles; n = 8) and nmf333 (filled circles, filled triangles, filled squares; n = 5 or 6) mice. P = 0.0009 between the 10⁶ CFU infected WT and nmf333 groups; log-rank test. (B) Number of B. cepacia recovered from the lung and spleen homogenates of WT and homozygous nmf333 mice 3.5 days after intratracheal inoculation with 10⁴ B. cepacia, as evaluated by quantitative culture; bacteria were not detected in lung and spleen homogenates from WT mice. Bars represent mean ± SEM (n = 3). **P = 0.029, *P = 0.043; t tests with 2 degrees of freedom. (C) Histopathological appearance of lung and spleen sections obtained from WT and homozygous nmf333 mice 3.5 days after intratracheal inoculation with 10⁴ CFU B. cepacia. Whereas WT tissue samples had minimal or no inflammation, necrotizing pneumonia and splenitis were apparent in the nmf333 strain. Hematoxylin and eosin staining were used. Scale bars: 100 μm.