Mutation of the Cyba gene encoding p22^phox causes vestibular and immune defects in mice
J. Clin. Invest. Yoko Nakano, et al. 118:1176 doi:10.1172/JCI33835 [Go to this article.]

Figure 2
Lack of p22phox expression and superoxide production in the neutrophil granulocytes of homozygous nmf333 mice. (A) Immunoblot detection of p22phox in the SDS-PAGE–separated protein lysate of WT neutrophils. The p22phox band is absent from the lysate of nmf333 neutrophils (top). Comparable protein loading is demonstrated by the actin signal present in both samples (bottom). Arrowheads and numbers indicate the positions of MW standards. (B) Rate of superoxide production by nonstimulated (–) and PMA-activated (+) neutrophil granulocytes isolated from the bone marrow of WT and homozygous nmf333 mice, as measured using the superoxide dismutase–sensitive cytochrome c reduction assay. Bars represent mean ± SEM (n = 3–5). ***P = 0.0005; unpaired 2-tailed t test. (C) Superoxide production of neutrophil granulocytes isolated from the bone marrow of WT (open circles) and homozygous nmf333 (filled circles) mice and stimulated with PMA (arrow), as detected by luminol-amplified chemiluminescence. Traces are representative of n = 3. RLU, relative light units.