Seven mutations in the human insulin gene linked to permanent neonatal/infancy-onset diabetes mellitus
J. Clin. Invest. Carlo Colombo, et al. 118:2148 doi:10.1172/JCI33777 [Go to this article.]

Figure 8
Annexin V expression and propidium iodide assay. (A) Percentage of apoptotic cells, as assessed by annexin V expression, in HEK293 cells transfected with human WT (pro)insulin or L^B15Y^B16delinsH mutation. Expression of mutant (pro)insulin effected a 3-fold increase in annexin V–positive cells 24 h after transfection compared with cells transfected with WT (pro)insulin. (B) Percentage of propidium iodide–positive cells transfected with WT, L^B15Y^B16delinsH, and C^A6Y mutation cDNA. P values are shown compared with WT (pro)insulin.