Seven mutations in the human insulin gene linked to permanent neonatal/infancy-onset diabetes mellitus
J. Clin. Invest. Carlo Colombo, et al. 118:2148 doi:10.1172/JCI33777 [Go to this article.]

Figure 7
Grp78 expression. Western blot with anti-Grp78 primary antibody in HEK293 cells transfected with WT and mutated insulin. Cytoplasmic fractions were collected at 9, 16, and 24 h after transfection. Expression of β-tubulin was assessed on the same filters to normalize the amount of loaded proteins. Increased Grp78 protein expression was clearly visible at 9 and 16 h for mutations L^B15Y^B16delinsH and C^A6Y and at 24 h for mutations L^B6P and L^B11P.