Seven mutations in the human insulin gene linked to permanent neonatal/infancy-onset diabetes mellitus
J. Clin. Invest. Carlo Colombo, et al. 118:2148 doi:10.1172/JCI33777 [Go to this article.]

Figure 5
Viability of transfected INS-1E cells. Shown is the viability of INS-1E cells at 48 and 96 h after transfection with WT human (pro)insulin, hAkita mutation, human familial hyper(pro)insulinemia mutation R65L, and human MDI mutations R65C, C^A6Y, L^B15Y^B16delinsH, L^B6P, L^B11P, and Y^A19X. Human (pro)insulin was detected by monoclonal antibody directed toward the human C-peptide and C terminus of the B-chain of the (pro)insulin molecule. Few (L^B6P and L^B11P) or no (hAkita, R65C, C^A6Y, L^B15Y^B16delinsH, and Y^A19X) INS-1E cells expressing MDI mutations were visible 96 h after transfection.