Tetraspanin TM4SF5 mediates loss of contact inhibition through epithelial-mesenchymal transition in human hepatocarcinoma
J. Clin. Invest. Sin-Ae Lee, et al. 118:1354 doi:10.1172/JCI33768 [Go to this article.]

Figure 3
TM4SF5 enhanced cytosolic p27^Kip1 stabilization. (A) Representative normal and tumor tissues for immunohistochemistry for p27^Kip1. Original magnification, ×400. SNU449Cp and SNU449Tp were double-stained with DAPI and either anti-p27^Kip1 (B) or anti-hKIS (D). (C) Lysates prepared as in Figure 1B were immunoblotted. (E) RT-PCR for p27^Kip1 or GAPDH by using total RNA prepared from actinomycin D–treated cells (Act D, 10 μg/ml; upper panel) or immunoblots for p27^Kip1 or α-tubulin using lysates from cycloheximide treated cells (CHX, 10 mM; middle panel) were performed. Lower panels: Band intensities were measured, normalized to control band intensities, and plotted. Scale bars: 20 μm.