Tetraspanin TM4SF5 mediates loss of contact inhibition through epithelial-mesenchymal transition in human hepatocarcinoma
J. Clin. Invest. Sin-Ae Lee, et al. 118:1354 doi:10.1172/JCI33768 [Go to this article.]

Figure 1
TM4SF5 overexpression in hepatocarcinoma tissues and SNU449 hepatocyte model system. (A) TM4SF5 overexpression in hepatocarcinoma. Normal (N1–N9) and tumor (T1– T9) tissues separately obtained from 9 hepatocarcinoma patients were analyzed by standard Western blots (upper panel) and by immunohistochemistry (lower panel). N6 and T6 were prepared only for immunocytochemistry, because the dissected tissue was too small. Six representative pair cases are shown for TM4SF5 immunohistochemistry. (B) SNU449 cells without or with TM4SF5. Parental (P) and stable SNU449 cells without (Cp) or with TM4SF5 expression (Tp, T3, T7, and T16) were imaged and immunoblotted. Original magnification, ×400 (A); ×100 (B).