J Clin Invest.
Quantitative assessment of ADK immunoreactivity and GFAP expression in the CA3 region.
Quantitative data were generated from KA-control, KA-SHAM, KA-WT, and KA-KO animals 3 weeks after KA injection/cell transplantation. (A) The relative density of ADK immunoreactivity was determined on DAB-stained sections by scanning CA3a fields of 300 × 200 μm on 2 sections each from n = 6 animals per treatment group. Data were normalized to the contralateral hippocampus of KA-injected control animals. (B) The total number of GFAP-positive cells in corresponding CA3a fields of 300 × 200 μm was determined by counting GFAP-positive cells on 2 sections each from n = 6 animals per treatment group. Data analysis was done by ANOVA; mean ± SD. **P < 0.01; ***P < 0.001.