Impaired microRNA processing causes corpus luteum insufficiency and infertility in mice
J. Clin. Invest. Motoyuki Otsuka, et al. 118:1944 doi:10.1172/JCI33680 [Go to this article.]

Figure 3
TIMP1 expression is elevated in Dicer^d/d female mice ovaries. (A) Mouse angiogenesis-related antibody arrays hybridized with ovarian lysates from Dicer^+/+ and Dicer^d/d mice on day 1.5 after coitus. Two independent experiments using different Dicer^+/+ and Dicer^d/d littermate mice pairs were performed. A representative image is shown. The positions of TIMP1 are denoted by boxes and arrows. The positive loading controls are also denoted by boxes and arrows. The map of antibodies spotted on these arrays is shown below. (B) Signal intensity ratios of each gene between Dicer^+/+ and Dicer^d/d mice ovaries obtained by protein array analyses. The normalized signal intensities of each protein from Dicer^d/d mice ovaries were divided by those from Dicer^+/+ mice ovaries. Four values for each gene obtained from 2 independent experimental sets were used for the analysis. The data are expressed as mean ± SD. Genes with low signal intensity that could not be analyzed further are expressed as N.D. (not determined). The mean signal intensities of TIMP1 in Dicer^d/d mice ovaries are more than 2-fold those in Dicer^+/+ mice. (C) The distribution of log₁₀ conversion values of the average of normalized signal intensities (n = 4) from each protein is shown as a scatter plot. The solid lines indicate the position of 2-fold changes in the intensities. The results for TIMP1 and platelet factor 4 are indicated by arrows. (D) Verification of Dicer1 protein deficiency and TIMP1 protein upregulation in Dicer^d/d mice ovaries. Dicer1 and TIMP1 protein expression in the ovaries of 2 separate Dicer^d/d and Dicer^+/+ littermate mice on day 1.5 after coitus are shown. GAPDH was used as a loading control.