BRAF gene duplication constitutes a mechanism of MAPK pathway activation in low-grade astrocytomas
J. Clin. Invest. Stefan Pfister, et al. 118:1739 doi:10.1172/JCI33656 [Go to this article.]

Figure 4
Functional analyses of low-grade glioma cell lines after pharmacological inhibition of MAPK signaling. Cell-cycle analysis by measurement of DNA content in NCH492 cells prior to (A) and 48 hours after treatment with 10 μM U0126 (C). Cells were arrested in G2/M upon treatment with U0126 and lacked a subG1-peak, indicating no increase in apoptosis. Early apoptosis rate was additionally determined by annexin V staining and was only slightly increased 24 hours after drug treatment (B and D). Puromycin was used as a positive control for apoptosis induction (E and F).