BRAF gene duplication constitutes a mechanism of MAPK pathway activation in low-grade astrocytomas
J. Clin. Invest. Stefan Pfister, et al. 118:1739 doi:10.1172/JCI33656 [
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Figure 1DNA copy-number aberrations in pediatric low-grade astrocytomas. (
A) Frequencies of DNA copy number gains and losses of tumors (
n = 66) were plotted against their chromosomal position. (
B) Complete array-CGH trace of a pilocytic astrocytoma, with a
BRAF duplication at 7q34 representing the only detectable genomic aberration in this tumor. This constellation was found in 13 of 66 (20%) tumors (arrow indicates clones with copy number gain). (
C) Array-CGH profile of chromosome 7 in another pilocytic astrocytoma with duplication of the
BRAF locus at 7q34 (arrow indicates clones with copy number gain). (
D) Duplication of the
BRAF locus in a pilocytic astrocytoma as assessed by FISH. The probe covering the
BRAF locus (3 copies) was labeled in green, and the centromeric control probe (2 copies) in red. Original magnification, ×100.
