Development of type 2 diabetes following intrauterine growth retardation in rats is associated with progressive epigenetic silencing of Pdx1
J. Clin. Invest. Jun H. Park, et al. 118:2316 doi:10.1172/JCI33655 [
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Figure 6Reversal of histone modifications partially restores
Pdx1 expression in IUGR islets.
The relative amount of acetylated histone H3 (
A), H3K4me3 (
B), and
Pdx1 mRNA (
C) levels measured by Q-PCR and normalized to
Gapdh.
n = 3 experiments, data are ± SEM; *
P < 0.05 versus controls;
#P < 0.05 versus pretreated IUGR islets. (
D) USF-1–bound
Pdx1 promoter, as detected by ChIP, was measured by Q-PCR and normalized to input DNA.
n = 3 experiments, data are ± SEM; *
P < 0.05 versus controls;
#P < 0.05 versus pretreated IUGR islets.
