Dopamine 5 receptor mediates Ang II type 1 receptor degradation via a ubiquitin-proteasome pathway in mice and human cells
J. Clin. Invest. Hewang Li, et al. 118:2180 doi:10.1172/JCI33637 [
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Figure 2Effect of Fen on AT
1R protein expression.
Immortalized human RPT cells were incubated with vehicle (lane 1), Fen (1 μM for 20 min; lane 2), or SCH 23390 (1 μM for 50 min) in the absence (lane 3) or presence (lane 4) of Fen. AT
1R protein expression in total cell lysates was determined by immunoblotting with anti-AT
1R (top panel): 2 bands were observed, denoted a and b, which presumably represent the glycosylated and nonglycosylated forms of the AT
1R, respectively (
17,
18). Immunoblotting for actin (bottom panel) showed equal sample loading in each lane. Shown is 1 immunoblot from 3 experiments. The effect of vehicle, Fen, or SCH 23390 in the presence or absence of Fen on AT
1R protein expression was also quantified.
n = 3. *
P < 0.05 versus vehicle, ANOVA, Student-Newman-Keuls test. Data are mean ± SEM.
