α3β1 integrin–controlled Smad7 regulates reepithelialization during wound healing in mice
J. Clin. Invest. Louise E. Reynolds, et al. 118:965 doi:10.1172/JCI33538 [Go to this article.]

Figure 4
Cytoplasmic translocation of Smad7 is increased in Itga3^–/– keratinocytes. WT or Itga3^–/– keratinocytes were treated with TGF-β1 for 0, 0.5, 1, 2, 3, or 7 hours, and cytoplasmic or nuclear extracts were analyzed by Western blotting for Smad7 (A), phospho-Smad2 (B), phospho-Smad3 (C), and Smad4 (D). Graphs represent mean (±SEM) densitometry results relative to mean HSC-70 (for cytoplasmic extracts, A) or PCNA (for nuclear extracts, B–D) loading control levels. n = 3–4 independent experiments. Solid line, WT; dashed line, Itga3^–/–. *P < 0.05.