α3β1 integrin–controlled Smad7 regulates reepithelialization during wound healing in mice
J. Clin. Invest. Louise E. Reynolds, et al. 118:965 doi:10.1172/JCI33538 [
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Figure 3Enhanced Smad7 expression in
Itga3–/– wounds in vivo and in vitro.
Smad7 levels (
A) and percentages of keratinocyte nuclei positive for phospho-Smad2 (
B) in 2-day
Itga3–/– and WT wounded epidermis. Western blot analysis of Smad7 protein levels in keratinocytes (
C). Western blotting for phospho-Smad2, phospho-Smad3, total Smad2, Smad3 (
D), and Smad4 levels (
E) in WT and
Itga3–/– keratinocytes treated with TGF-β1 (+) or left untreated (–). TGF-βRI levels in WT or
Itga3–/– keratinocytes (
F). HSC-70 provides relative loading controls. Bar charts in
C–
F represent densitometry results (mean ± SEM) from 3 independent experiments. *
P < 0.05.
