α3β1 integrin–controlled Smad7 regulates reepithelialization during wound healing in mice
J. Clin. Invest. Louise E. Reynolds, et al. 118:965 doi:10.1172/JCI33538 [
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Figure 2TGF-β1–mediated migration is reduced in
Itga3–/– keratinocytes.
Confluent cultures of WT or
Itga3–/– keratinocytes were scratched, either in the absence (–) or presence (+) of 1 ng/ml TGF-β1, and changes in the rate of scratch closure were observed 24 hours after wounding (
A). Results in
A represent the mean scratch width at 24 hours relative to the scratch width at 0 hours (%) ± SEM;
n = 3 separate samples/genotype/treatment. Single-cell migration of WT and
Itga3–/– keratinocytes in the absence or presence of TGF-β1 on Coll IV, Fn, Lm 5, or no ECM (
B). Results in
B represent the mean migration of single cells (±SEM) relative to untreated WT controls.
n = 3 experiments. *
P < 0.05.
