RelA/p65 promotes osteoclast differentiation by blocking a RANKL-induced apoptotic JNK pathway in mice
J. Clin. Invest. Sergio Vaira, et al. 118:2088 doi:10.1172/JCI33392 [
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Figure 2Rela–/– RCs are protected from arthritis-induced bone loss.
(
A) Arthritis was induced in
Rela+/+ and
Rela–/– RCs 5 weeks after marrow transplantation by injection of K/BxN serum at days 0, 2, and 7, with LPS on day 2. Histological sections of ankle joints at day 14 stained with H&E show an extensive and similar inflammatory infiltrate in the joint spaces. Scale bar: 500 μm. (
B) The inflammatory response was also evaluated by measurement of hind paw thickness (sum of both paws) during induction of arthritis.
n = 8/group. The experiment was repeated twice, with similar results. (
C) TRAP-stained sections of metatarsals demonstrate OCs both on the outer bone surface adjacent to inflammatory pannus (arrowheads) and in marrow spaces. Scale bar: 100 μm. (
D) Histomorphometric analysis of N.Oc./mm and Oc.S./BS. on the outer bone surface demonstrate significantly more OCs in
Rela+/+ RCs compared with
Rela–/– RCs. *
P < 0.001;
n = 8/group. (
E) Serum CTX was measured at day 0 and 14 of serum transfer arthritis, and the fold change for each RC was plotted, demonstrating a significant rise only in the
Rela+/+ group.
†P < 0.05;
n = 6/group.
