Figure 1
Design of antisense morpholinos.
(A) Inclusion of ClC-1 exon 7a induces a frame shift and premature termination codon in exon 7. Annealing of antisense morpholino to the 3′ splice site of exon 7a in the ClC-1 pre-mRNA is intended to prevent spliceosomal recognition of this exon. (B) Alignment of ClC-1 pre-mRNA (top strand) with antisense morpholinos targeting the 3′ or 5′ splice sites of exon 7a is shown. The control morpholino is the 5′-3′ invert of the 3′ splice site blocker. Exonic sequences are in upper case, intronic sequences are in lower case.
