The type I IFN induction pathway constrains Th17-mediated autoimmune inflammation in mice
J. Clin. Invest. Beichu Guo, et al. 118:1680 doi:10.1172/JCI33342 [Go to this article.]

Figure 5
The TRIF pathway limits Th17 development through induction of antiinflammatory cytokine IL-27. (A) WT and TRIF-deficient macrophages were stimulated with 100 ng/ml LPS. The amount of IL-27 protein in culture supernatants was measured after 24 hours of stimulation. (B) WT and TRIF-deficient DCs were stimulated with 100 ng/ml of LPS. The amount of IL-27 protein was measured after 24 hours of stimulation. (C) IL-27 inhibits IL-17 production induced by IL-6 and TGF-β. Naive CD4⁺ T cells isolated from WT mice were activated for 72 hours in Th17 culture in the presence of IL-27 as indicated. IL-17 production by CD4⁺ T cells was determined by ELISA. (D) IL-27 inhibits antigen-induced Th17 response. Total splenocytes isolated from WT mice 21 days after MOG/CFA immunization were restimulated with MOG peptide ex vivo in the presence of IL-27 for 3 days. IL-17 production was measured by ELISA. Data shown are representative of at least 3 experiments. (E and F) WT and IFNAR^–/– BMMs or DCs were stimulated with 100 ng/ml LPS. The amount of IL-27 protein was measured after 24 hours of stimulation.