A contrast agent recognizing activated platelets reveals murine cerebral malaria pathology undetectable by conventional MRI
J. Clin. Invest. Constantin von zur Muhlen, et al. 118:1198 doi:10.1172/JCI33314 [
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Figure 6Histology of TNF-injected brains with LIBS-MPIO injection. (
A) Cresyl-violet stain revealed binding of MPIO to areas on the vascular wall (arrows). (
B) Binding of LIBS-MPIO to platelets or platelet thrombi was confirmed using immunohistochemistry for platelet-specific CD41: 2 MPIOs appearing in different focus and therefore of different shape were recognized at areas of platelet aggregation (arrows). (
C) Similarly, MPIOs were detected on platelets and platelet thrombi in animals with CM (inset, high-power view of the region indicated by arrow). (
D) Platelet-positive elements per injected hemisphere at 6, 12, and 24 h after intracerebral injection of TNF (diamonds) or saline (squares) in mice. (
E) Quantification of the LIBS-MPIO–induced signal void in animals 6, 12, and 24 h after intracerebral TNF injection. Note the correlation between the number of platelet-positive elements over time and the LIBS-MPIO–induced signal void (
r2 = 0.9503).
