The transcription factor IFN regulatory factor–4 controls experimental colitis in mice via T cell–derived IL-6
J. Clin. Invest. Jonas Mudter, et al. 118:2415 doi:10.1172/JCI33227 [Go to this article.]

Figure 9
Analysis of cytokine expression and apoptosis in the colon of reconstituted mice. (A) Expression of cytokine mRNA levels in the colon of RAG2-knockout mice reconstituted with WT or IRF4-deficient T cells was performed by quantitative PCR. Whereas mucosal IL-6 expression was significantly higher (**P < 0.01) in RAG2-knockout mice reconstituted with WT but not IRF4-deficient cells, no significant differences were noted in TNF and TGF-β levels. One representative experiment out of 3 is shown. (B) In addition, TUNEL assays on colonic cryosections were performed to detect apoptotic LPMCs. There was a significantly higher number of apoptotic LPMCs in the colons of mice reconstituted with IRF4-deficient T cells as compared with mice given WT T cells. Original magnification, ×200.